Detection of blaCTX-M gene among Pseudomonas aeruginosa isolated from water samples in Baghdad

Abstract

A total of 50 environmental Pseudomonas aeruginosa isolates were collected from sewage and tapwater in Baghdad, Iraq. The MICs of Cefotaxime and Ceftazidime were determined by using agardilution method, The MIC ranged from 2 to 256 μg/ml.The results of antibiotic sensitivity testshowed that among sewage P. aeruginosa isolates, resistance was observed most often toTicarcillin (92%), Penicillin G (84%), Ceftazidime (12%), (8%) for each of Cefotaxime andTicarcillin. On the other hand, all tap water isolates were sensitive to Ofloxacin and Levofloxacin,Except (5%) of isolates were resistant to Cefotaxime (25%) to Ceftazidime and (95%) toTicarcillin. All isolates were tested for Extended-Spectrum β-Lactamase (ESBL) production. Tenisolates (20%) were found to be ESBL producers. All environmental P. aeruginosa isolates werescreened for the presence of the blaCTX-M genes by application PCR, Only (30%) of them werepositive for this test.