دراسة مقارنة لأنواع مختلفة من بكتريا Mycoplasma بطريقتي العزل الجرثومي MDCS وال PCR

Abstract

AbstractA 150 samples were collected (50 samples of sputum , 50 swabs of the gingiva and 50 of vaginal swabs) from patients who admitted Al -Basra general hospital and the specialist center of the first dental medicine in the governorate of Basra for the period from January 2015 to May 2015 from males and females , their age of patients ranged from 6-70 years old . Samples were collected and96cultured by monophasic-diphasic culture setup method ( MDCS ) .Three types of Mycoplasma were isolated : Mycoplasma pneumoniae from sputum , Mycoplasma salivarium from the swabs of the gingiva and Ureaplasma urealyticum from the vaginal swabs. Isolated Mycoplasma were diagnosed by biochemical tests and PCR technique .Results were statistical analysis by chi – square at P ≤ 0.05 level . Mycoplasma spp . were isolated from 76 individuals out of 150 samples with biochemical tests. Twenty five cases were diagnosed as M. pneumoniae (50%) , 13 cases were diagnosed as M. salivarium (26%) and 38 cases were diagnosed as U.urealyticum (76%) .Sixty eight isolates of Mycoplasma spp. were diagnosed by PCR at first time in Iraq , 23 were diagnosed as M. pneumoniae (46%) , 13 were diagnosed as M. salivarium (26%) and 32 were diagnosed as U. urealyticum (64%).