Molecular assessment of apoptotic genes in leukemic patients

Abstract

AbstractLeukemia is one of the commonest ten cancers in Iraq and worldwide. This disease is classified into myeloid or lymphoid and they are further divided into chronic or acute leukemia. This research included chronic myeloid leukemia (CML) in fourty nine patients (22males and 27females).Multiplex polymerase chain reaction was done to analyze five human apoptotic genes (P53,Bcl2,Cmyc,ICE and GAPDH).These genes demonstrated highly significant comparing with ten control p<0.05(p=0.000, p=0.000, p=0.000, p=0.004) GAPDH regarded as internal control. While they didn’t show any significance with gender, age groups or phases except P53 was showed highly significant with blast transformation phase. These results showed that genes were expressed in patients, and these may regard as important indicators for this disease. Further studies are needed to confirm this observation and better elucidate the mechanism. The aim of this research is to study the molecular expression of apoptotic genes in leukemic patients compared with healthy controls and study the expression of apoptotic genes in leukemic patients related to gender, age groups and phases of CML.