Preparation of Vaccine for pathogenic bacteria causing abortion using low level diode laser

Abstract

The objective of this study was investigate the effect of Low Level diodelaser on the bacterial isolates (Listeria monocytogenes, Enterobacter cloacae,Klebsiella pneumonia, Staph aureus, Esherichia coli and Pseudomonasaeruginosa) which used in preparation of vaccine and to determine the immuneresponses using the RID kit. The antibacterial agent’s sensitivity before irradiationwas performed by using twelve antibiotics, these strains showed high resistance forthese antibiotics except amikacin. But after irradiation the sensitivity wasincreased. Some features were studied such as bacterial count which decreased andlost of blood hemolysis and biocyanine stain from p.aeruginosa according toincreasing the time and power of irradiation. Bacterial growth killed and attenuatedto prepare vaccine using diode laser (660) nm of wavelength having (50 and 250)mw of power and the frequency (1-10) kHz. The experiment was conducted on 24adult white New Zealand male rabbits with (1.5-2) Kg body weight each, theywere divided into sixth groups with 4 rabbits each and inoculated with killed andattenuated vaccine and one group was used as control. After (35day) ofimmunization, determination was done for (IgM, IgG, IgA in addition to C3 andC4). The Ig and Co. of immunized animals were higher (P>0.05) compared withcontrol animals, also the live attenuated vaccine induced highly immune responseas compared with killed vaccine. The control group died after challenge dose whilethe immunized animals not.