Estimation of Antibacterial Activity of Green Mango (mangifera indica L.) Extract on the Growth of Bacteria

Abstract

Alternative herbal medicines have been used since hundreds of years in globe in treatment of various kinds of infections that may infect human as well as animals. Medicinal plants, in general, contain phytoconstituents some of which having chemical properties resemble to that of synthetic antibiotics. Due to this, the aim of this study is to investigate the antibacterial activity of the green mango (mangifera indica L.) extract against both Gram-negative bacteria and Gram-positive bacteria. The extraction process of the active phytoconstituents of green mango was done by using infusion method. The estimation of antimicrobial activity of green mango extract was carried out by using agar well diffusion method against two species of gram-positive bacteria and five species gram-negative bacteria using three concentrations that are 5%, 10% and 50% of the extract. The 5% concentration of the extract has showed 2 mm diameter inhibition zone against both Proteus and Salmonella, 5mm diameter inhibition zone against Pseudomonas, 7mm diameter against both Klebsiella and Staphylococcus, and 11, 12 mm diameter inhibition zone against E.coli and Streptococcus respectively. The 50% concentration of extract has showed 14 mm diameter against Proteus, 19, 20, 20mm diameter against Salomella, Klebsiella and Staphylococcus respectively, 22, 23 mm diameter against E.coli and Pseudomonas respectively and 26 mm diameter against Streptococcus. Lastly, the 10 % concentration of extract has showed a result ranged between the 5% concentration and 50% concentration.