Comparison of Antimicrobial Activity of Olive Leaves Extracts and Apple Cider Vinegar against Bacterial isolates Obtained from Otitis Media

Abstract

The present study included collected 120 sample from patients with otitis media, who attended to consultants of Ear nose throat in AL-Hakeem hospital in Najaf city. Through the period from (8/10/2017-8/1/2018). The results revealed that the Pseudomonas aeruginosa was the most common bacterial isolates (45) 37.5%, followed by Staphylococcus aureus & Acinetobacter baumannii which constituted (25) 21%, (16) 13% respectively. Test antimicrobial activity of olive leaves extracts and apple cider vinegar against pathogenic bacteria causing otitis media using agar well diffusion method. This result indicated a significant effect (p < 0.05), among the microorganism Proteus sp which showed most sensitive to cold olive leaves extract range 10-28mm, both Staphylococcus aureus and Pseudomonas fluorescence showed to high sensitivity boiled olive leaves extract range from 14-28mm, while Pseudomonas aeruginosa & Proteus sp at high levels of inhibition range 15-30mm. in comparison apple cider vinegar The maximum inhibition zone of Gram negative bacteria was observed against Escherichia coli, Acinetobacter baumannii 20-37mm, another bacteria include (Pseudomonas aeruginosa, Pseudomonas fluorescence) & Proteus sp the inhibition zone range 15-35mm & 15-30mm respectively, while the observed maximum inhibition zone of Gram positive bacteria 15-30mm, This study shows that Apple cider vinegar consider more effect in the control of otitis media pathogens than olive leaves extract.