Production of single cell protein from growth local Candida utilis, Candida tropicalis tropicalis Isolates on Whey and remnants of dates

Abstract

This study was conducted for producing a single-cell protein from Whey andremnants of dates, using local yeast Isolated.The efficient Isolates were selected forutilization of the used carbon sources and production of single-cell protein.The limitationof the appropriate incubation period for growth and production as trial to improving thenutrient media components which was performed by the increment of the prepared ofcarbon source by focusing Whey or mixing it with the remnants of dates at differentrates. The addition of supports to the media included food powder plant Northblan andremnants of culture of mushrooms and shellfish waste of bloody massacres meat andcrude oil.The study also tackled the impact of the use of selected mixed culture isolatesand impact of shaking and aeration processes on producing single-cell protein.Theproduction of single cell protein was performed in shaking incubator and by usingFermenter.The most important findings are :Sexes Isolates obtained from natural sources, which included 3 Isolates of thewhey environment and 3 Isolates of remnants of dates.Test results showed the efficiencyof isolates selected in the production of single cell protein fermentation process for fourdays, the concentrated whey media gave the best production rate 4.60 g / liter. The studyshowed that mixing 50% of remnants of dates with 50% of concentrated whey led tosignificant increase in the production was 6.56 g / liter. The use of the improvedmaterials for the media led to significant increase in the results, achieving higherproductivity 8.35 g / liter when using remnants of the bloody massacres of meat. The useof mixed cultures led to significant increase in the production compared with purecultures of Isolates that gave mixed isolates Wt2Dr1Fl higher productivity amounted to14.79 grams / liter. The study showed that the process of agitation and aeration hadsignificant role in raising production to hit 17.61 grams / liter when using aeration rate of0.5 liter / minutes with shaking speed of 150 cycle / minutes.. The use of the Fermenterinstead of the shaking incubator had significant increased production rate of the singlecellprotein that was 23.35 grams / liter. The results of diagnostic tests showed thatisolates returning to Ascomycetes, Genus of Candida, as Candida utilisWt2 and C.tropicalisDr1.The analysis of results showed that the components of single-cell proteinproduct that the proportion of crude protein ranged between 52.55 - 57.01%, andcarbohydrates 23.74 - 26.9%, and the proportion of ether extracted fat ranged 2.74 -2.98%, the percentage of nucleic acid (3.40-3.96%). The study showed a decline in thevalue of BOD for the used mediato 480 mg / liter at the development mix isolatesWt2Dr1Fl .