Relationship of Seminal plasma Total Antioxidant Capacity (TAC) with LH and FSH Levels in Serum with Sperm Function Parameters in Different Groups of Infertile Men

Abstract

Background: Oxidative stress (OS) plays a vital role in human reproduction. Itrises as a consequence of excessive reactive oxygen species (ROS) productionand/or reduced total antioxidant capacity (TAC) protection. Oxidative stress mediateddamages to the plasma membrane of the spermatozoa may account for defectivespermatozoa function parameters that are observed in a high percentageof infertile patients.Objectives: This study aimed to evaluate the relationship between level of seminalplasma TAC in relevance to luteinizing hormone (LH) and follicle stimulatinghormone (FSH) in serum with the assessment of routine spermatozoaparameters in different groups of infertile men.Subjects,Materials and Methods: Blood and semen samples were collected fromthe infertile and fertile subjects. The total number of samples were (80), the infertilemen were divided into three groups (azoospermic, oligozoospermic andasthenozoospermic) each with (20) sample, and (20) as fertile men. Then the levelsof serum hormones and seminal plasma TAC were measured using EnzymeLinked Immunosorbent Assay (ELISA).Results: The results of hormonal levels in serum showed that there were highlysignificant difference (P<0.001) in the levels of FSH and LH between controlgroup and infertile groups. In this study its appear that there were a highly significant(p<0.001) difference in the level of TAC between study groups and controlgroup which were increased among normozoospermic men and decreased amonginfertile groups. The lower limit of TAC were seen in asthenozoospermic men.The results of this study also showed that there were a relationship between TACof seminal plasma and hormonal levels, it is appear that TAC are negatively correlatedwith LH and FSH.Conclusions: From the results obtained it could be concluded that; LH and FSHhave an effect on male fertility via regulation of seminal plasma TAC. On theother hand seminal plasma TAC are negatively correlated with the number of immotilespermatozoa and positively correlated with concentration, progressivelymotile and morphologically normal spermatozoa.