Assessment Of Lipid Peroxidation And Glutathione Levels In Serum And Seminal Plasma For Unexplained Infertile Men In Thi-Qar Governorate

Abstract

Infertility is the inability of a sexually active, non-contracepting couple to achieve spontaneouspregnancy in one year. unexplained male infertility (UMI) is reserved for infertile of unknown originand accounts for 6-27% of infertile men with normal semen analyses on two or more occasions withno demonstrable physical or endocrine abnormalities and female factor infertility has been ruled out.Oxidative stress (OS) is a consequence of an imbalance between the production of reactive oxygenspecies(ROS) and the body’s antioxidant defense mechanisms. However oxidative stress(OS) is acommon pathology seen in approximately half of all infertile men. It has been shown that reactiveoxygen species (ROS) can lead to deleterious effects on a range of sperm parameters. The monitoringof MDA levels in different biological systems can be used as an important indicator of lipidperoxidation .Humans have developed a highly ,organized and complex antioxidant defense systemto protection cells and organ systems of the body against reactive oxygen species(ROS) such asglutathione is capable of reducing ROS levels and lipid peroxidation..Serum and seminal plasma MDA was significantly increased in unexplained infertile(smoker andnonsmoker)groups when compared to fertile (smoker and nonsmoker)groups. The serum andseminal plasma glutathione were significantly decreased in unexplained infertile( smoker andnonsmoker)groups when compared to fertile (smoker and nonsmoker)groups. The decrease inantioxidant levels appears to be mainly a consequence of increased oxidative stress.