Investigation of Clostridium perfringens in inflamed wounds and gauze of the patients′ feet infected with diabetics

Abstract

One hundred samples were collected during the study of diabetic foot section of Al-Sadr city hospital medical supplies for the period from 16/5/2017 to 15/8/2017. The bacteria were first grown on the medium of the thyoglycolate broth using anaerobic conditions and several liberalization of gas [Gas pak] were then grown bacteria on Glucose- Blood agar It has been isolated [20] Clostridium perfringens bacteria isolated belonged to distributed among [19] isolation of diabetic foot wounds and isolate one of the gauz. Through morphological and biochemical tests and culture media of proved it Clostridium perfringens as revealed through microscopic examination ,it is Gram positive with bacilli shape and non -motile anaerobic, spores forming more over the shapes of spores was oval sub-terminal. Bacteria were grown in Litmus Milk medium gave test positive through fermentation stormy. The results of biochemical tests showed they are unproductive enzyme Catalase and Oxidase as it gave a test negative and it turned out that it is positive for the degradation of gelatin as it shows that have the ability to secrete the enzyme Gelatinase. it also that is a starch hydrolysis through the secretion of the enzyme Amylase, Ferment glucose, dextrose, fructose, lactose and galactose while non- ferment mannitol and salicin.