Evaluation of the activity of alcoholic extract against Hibiscus sabdariffa L. against biofilm formation of Candida albicans

Abstract

In the current study , it was highlighted to find out the inhibitory effect of the alcoholic extract of Hibiscus sabdariffa against Candida albicans isolated from mouth area and around teeth through the period from 2017/12/1 until 2018/2/27 . Different concentration of alcoholic extract of Hibiscus sabdariffa were tested on the yeast C.albicans by well diffusion method and microdilution method . The results showed that concentrations (50 , 100 , 150) mg/ ml of the crude extract gave inhibition zone diameter rates ( 23 , 27 , 30) mm respectively, and the minimum inhibitory concentration (MIC ) was (25) mg/ml against the yeast cells. Concentrations (12.5 , 25 , 50) mg/ ml were used to study the activity of the extract on biofilm, The results showed the inhibitory effect of the extract on biofilm . From the current study , we concluded that, the high efficiency of alcoholic extract of Hibiscus sabdariffa against the yeast C.albicans, suggesting the possibility of its use as a treatment for patients with Candidiasis .