Role of 1α-Hydroxylase Enzyme and 25(OH)D as a Biomarker of Vitamin D Metabolism in Chronic Kidney Disease

Abstract

Chronic kidney disease is identified as a risk factor for vitamin D deficiency, vitamin D deficiency is common and high in the chronic kidney disease population so it is recognized as a significant public health problem. The objective of this study was to 1) measured 25(OH)D and 1α-hydroxylase levels. 2) Correlation between 25(OH)D and 1α-hydroxylase among patients with vitamin D deficient and CKD. Blood samples were collected, from 50 patients suffer from CKD and have Vitamin D deficiency, and 50 healthy persons as control group. The biochemical results showed that blood urea, creatinine, phosphorus and parathyroid hormone levels a highly significant increase in the level of patients compared with controls. The biochemical results showed that blood urea, creatinine, phosphorus and parathyroid hormone levels were highly significant increase in the patients compared with controls. While the results revealed a highly significant reduction (P < 0.01) in 25(OH)D level, calcium and 1α-hydroxylase enzyme among CKD patients when compared with the healthy controls. The biochemical markers showed a highly significantly correlation between serum 25(OH)D and Creatinine, phosphorus and parathyroid hormone (P<0.01). While a highly significant reduction correlation was observed between serum 25(OH)D and calcium, serum 1α-hydroxylase enzyme levels in CKD patients.