Determine the Antibacterial Activity of Staphyloxathin Produced by Staphylococcus aureus against some Bacteria

Abstract

A total of 70 isolate of staphylococcus (22.8%) isolated from 306 sample of different clinical sources that is diagnosing as S. aureus depending on cultural morphological and biochemical test. These characteristics include; colonial morphology, size of colony, color and the effect on the media such as blood hemolysis, pigments appear on Milk agar and ability to ferment mannitol. Staphyloxanthin pigment was extracted by using different methods included procedure one using chloroform, procedure two using methanol and procedure three using ethyl acetate with ethanol was the best method. Staphyloxanthin purified by using coolum chromatography, purified extract of Staphyloxanthin that isolated from S. aureus as anti-bacterial activity ,which appeared inhibition zone against several gram negative bacteria at concentration (2,20)mg/ml , and the highly inhibition zone (15mm ) of pigment was recorded against Pseudomonas aeruginosa.