Study the bacteriological quality of bottled water in Baghdad province and Inhibitory effect of bacteriocin extracted from Pseudomonas aeruginosa

Abstract

Three hundred and nineteen samples of bottled water belonging to ten different local and imported brands (37 imported and 282 local samples ) collected from the Iraqi (in Baghdad ) market with different size ranged (500 ml to 20 litter ) during January tile June 2014 was analyzed bacteriologically.Heterotrophic plate count (HPC) was determined using R2A agar culture medium also all the collected samples were analyzed for the presence of coliform bacteria, fecal coliform bacteria, E. coli ,and, P. aeruginosa. Around 5 % of the water samples exhibited HPC counts ranged between 5- 500 cfu/ml.The bacteriological tests of water showed that the bacterial failure percentage was 5% of bottled water samples, the most probable number of total coliform ranged between 1.1 to 23 CFU/ 100ml. These results didn’t agree with national and international standard characters that detected no coliform bacteria / 100 ml of treated water.In this study three isolates of Pseudomonas aeruginosa ( most dominant bacteria) were tested for bacteriocin production and antimicrobial activities were measured by using the agar well diffusion method on some Gram positive and Gram negative pathogenic bacteria which involved (Aeromonas hydrophilia, Escherichia coli, Staphylococcus aureus, Streptococcus faecalis) were used as indicator isolates. Three isolates of Pseudomonas aeruginosa were positive for bacteriocin production with a wide range effect on gram positive and negative bacterial growth, with diameter (2-20) mm.