Isolation of microorganism from acute diarrhea in children below 5 years and Comparison the inhibitory effect of natural honey and some types of antibiotics against bacteria isolated

Abstract

The diarrhea is one of the most important disease infected children and lead them to enter the hospitals and increased vulnerability of our children to infection .In this study collect 25 stool samples from children the maternity and child hospital in Babil governorate by using clean and sterile cup . Then take samples to the microbiology laboratory to investigate the bacterial species causing diarrhea by cultured on the appropriate media and diagnostic biochemical tests were carried out. Present 16(36%) samples were positive obtained obtain from 25 samples belonging to the species of the following bacteria klebsiella pneumonia, Enterobacter, staphylococcus aureus, Enteropathogenic, E.coli, pseudomonas aeruginosa, Proteus. The effect of 10 types of antibiotic Cefixime, Amoxacillin Penicillin, Ceftriaxone, Erythromycin, Gentamycin, Streptomycin Trimethoprim + Sulfonamides, Nalidixic Acid, Imipenem was studied by using agar disc diffusion method . It was found that the Imipenem antibioticis most effective by affecting on all the bacterial isolated and least effective was Nalidixic acid. The most sensitive bacterial species were Enterobacter sp., Which were sensitive to all antibiotics and the most resistant were the proteus bacteria . When studying the effect of natural honey in inhibition of bacterial growth it was found to have a inhibitioneffect in preventing the growth of bacteria causing diarrhea similar the effect of antibiotic .