Study of Bacteria in Stool and Urine Among Food Handler In Ramadi City

Abstract

Abstract:Back ground: The carrier is a state which follow either symptomatic or asymptomatic salmonella infection. It may be temporary or chronic. Salmonella spp. can be detected by stool or urine cultures and serologic techniques. Aim of study: Bacteriological study (isolation and identification) of stool and urine among food handler in Ramadi city.Materials and Methods: One hundred (100 ) apparently healthy food handlers with no significant past medical history; 75 were restaurant and cafeteria workers while 25 persons were house wives. From each individual two specimens(stool, urine) were taken and inoculated immediately in selenite broth, incubated for 18 hours at 37oc and then sub-cultured on MacConky agar, Salmonella-Shigella agar, Xylose-Lysine Deoxycholate agar, and Bismoth sulfite agar plates and they were incubated for 24 to 48 hours at 370 C, a suspected salmonella spp. Colonies were identified according it's specialized methods.Results: The age of the studied individuals ranges from 16-52 years with mean age (27 + 8) years . Both urine and stool culture , all of them were negative for salmonella spp. Three specimens (( 3%)) of urine showed growth of E. coli while seven stool specimen were showing positive culture for other than salmonella organisms, E. coli showing 45% , Proteus spp. 30% , Citrobacter freundii 15% and Klebsiella spp . 10%.Conclusion: In this study all samples whether stool or urine were negative for salmonella species. So studied food handlers individuals were not carriers for salmonella in Ramadi city .