Production of Glucose isomerase from local Isolate of Streptomyces sp. HM5 2-Optimization of enzyme production conditions by submerged cultures

Abstract

This study was aimed to investigate the cultural requirements for production of glucose isomerase by locally isolated Streptomyces sp.HM5The optimum conditions for production of the enzyme by submerged culture was achieved on broth medium containing 1.5% of xylose as a sole carbon source and inducer for enzyme production , peptone with 1% as a nitrogen source and 0.05% of each one of magnesium sulfate and cobalt chloride as a metal ions source with an initial pH of 7.5 during an incubation period of 72 hours at 35°C.This optimum conditions for enzyme activity attained 9.59 unit/ml it means the enzyme activity increased about 192% in comparation with those before using optimum conditions.