Spectrophotometric Determination of Vitamin B1 (ThiaminHydrochloride)In Pharmaceutical Preparation by CouplingReaction with Diazotized Sulfanilic acid

Abstract

The experiment was conducted during the proied of 2009- 2010. Seeds were sterilized with 4% sodium hypochloride for 6 minuts, and sown on MS medium free of sucrose, supplemented with different concentration of liquorice extracts ( 25, 50, 75, 100 and 125 ml / l ). Cultures were incubated at 25ْ C ± 2 and 16 hr / day photoperiod, and 1000 Lux light intensity for 35 days. Produced plantlets were prepared into 1- 2 cm microcutting and transferred to the same medium. The first experiment comprises of different concentration of liquorice and cultivar to determine germination percentage, leaf number, shoot number, node number per plant, plant height and root length. The second experiment, included the effect of same concentration and the microcuttings on developed cuttings percentage, leaf number, shoot number, plant length and root length. Results showed that, the interaction of the conc. and cultivare had a significant impact on the characters studied. 100 m/l with cucumber Alghazer cv. gave the highest percentage of germination and the highest mean of root number reached ( 99.91% and 3.39 cm ) respectively. On the other had, the same concentration and empirtor cv. of cucumber highest means of leaf number, shoot number, node number and plant height ( 9.71, 3.91, 7.31 and 10.46 cm) respectively. The interaction of concentration and the cuttings showed a significant effect on the traits studied. 100 m/l with cucumber cuttings of cv. Alghazer gave the highest percentage of the developed cuttings and root length ( 99.37% and 2.01cm) respectively, while 100 m/l with empirtor cv. of cucumber resulted in higher means of leaf number, shoot number, node number/ plant and plant length ( 7.34, 2.22, 3.99 and 4.81 cm ) respectively.