Effect of salt stress on shoot multiplication of Mentha piperita L. in some biochemical character , and menthol production in vitro .
Abstract
An experimental was carried out to study the of peppermint plant () by tissue culture technique. The effect of different concentrations of NaCl (0 , 2 ,4 , 6, 8 ) g/ l ed to MS medium on multiplication rate of Mentha piperita shoots and on some biochemical properties In vitro produced shoots .The Results showed that the lower concentrations of NaCl ( 2 g/ l ) in culture medium caused a significant increase in multiplication rate ; fresh and dry weights of shoots , chlorophylls, potassium ions and menthol content , compared with the control treatment and higher concentrations of salt .Concerning the multiple shoots content from sodium, chloride , proline and carbohydrates these constituents were increased when the concentrations of NaCl increased in culture medium
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