Study of anti mutagenic and anti oxidant activity of clove plant extract in male white rats

Abstract

This study was designed to test the antioxidant and antimutagenic activity of methanolic water extract of clove. The extract has been described by using thin layer chromatography TLC, the results which showed the presence of more than one component of this extract and when examining the activity of antioxidant by beta carotene spray process , It was observed appearance of the antioxidant activity in several bands . The antioxidant activity was determinated for these bands through the retention of the color yellow, which indicates the existence of effective antioxidant in some components of clove extract. When rats treated with 2 mg/kg of MMC lead to increased the level of DNA fragmentation and the interference treatment of extract at 500 mg/kg (with) and (after) the mutagen performed decrease the level of fragmentation,Thes results may indicate that the extract works as desmutagenic agent, while when given befor the MMC, the extract may works as antimutagenic agent. So the result of the oral gavage of mixing the extract with mutagen for five weeks reducing the level of the crash in the DNA compared with negative control. The results of MI of the interference treatment with extract (with) and (after) the mutagen performed a best result in the raising of median MI to 2.43 , 2.13 respectively compared with the positive control 1.16, while the extract before the treatment with mutagen did not posses any significant differences 1.68 compared with positive control .The result of the oral gavage of mixing the extract with mutagen for five weeks had been showed, in the first week led to raise the median MI. The result of chromosomal abnormality CA of mice stem cell possessed a high significant difference in the positive control 26.0 compared with the negative control 5.33, while in the interference between the extract and mutagen the result showed a decrease in the median of abnormality to both treatment (with) and (after), while the treatment ( before) did not posses any significant difference compared with positive control . Also the results of median chromosomal abnormality of the interference treatment (with) to five weeks showed a decrease in the median of CA. The results of this study refer to that the clove extract have antioxidant and antimutagenic activity In vivo and In vitro.