Reducing Bacterial Strains from Polluted Water Using Zizyphus Spina

Abstract

Ziziphus spina leaves alcohol extract in concentrations of 250, 500, 750 and 1000 ppm and aqueous infusion of leaves in concentrations of 20000, 40000, 60000, 80000 and 100000 ppm were used to reduce Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus and total bacterial account from wastewater in laboratory conditions of 37 oC, pH 6.5 and 5-48 hr treatment period. Concentrations of leaves alcohol extract 250-1000 ppm showed high ability to reduce the bacteria Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus account by 36%-100% in 18 hr period treatment, while, leaves aqueous infusion in concentrations of 40000-100000 ppm showed its ability to reduce Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus by 34%-93% in 24 hr period treatment. Leaves alcohol extract in concentration of 1000 ppm showed high ability to reduce Escherichia coli and Staphylococcus aureus by 100% and TPC by 63% in 18 hr while leaves infusion 100000 ppm reduce the same bacteria by 100% and TPC by 28% in 48 hr treatment period.