Evaluation of Crude Anthrax Protective Antigen as an Adjutant For Brucella Abortus Vaccine In Mice

Abstract

Anthrax crude protective antigen (PA) was used in this study as an adjutantwith Brucella abortus S19 strain vaccine in a ratio of 1:1 to immunize mice indose of 0.1 ml s/c in order to decrease side effects of S19 vaccine and also toenhance its immunogenicity. Three groups of mice were immunized with themixture (composed of anthrax PA +S19, S19 alone, and sterile PBS solution asa control. They were compared concerning cellular and humeral immunity. Thegroup treated with the mixture was the best regarding skin test, the difference ofthickness was 1.51± 0.103 mm after 24 h and 1.14± 0.103 mm and 0.89± 0.100mm after 48 and 72 h, respectively. As well as that group revealed the highestantibody titer, which reported 0.7153± 0.08 nm as compared with 0.3743± 0.06nm in S19 group. These findings were verified by studying thehistopathological changes.