Using of Brucellins and Their Fractionation Peaks in Immunization Against Brucellosis

Abstract

A number of criteria have been considered during this study. These criteria include preparation and fractionation of brucellins using chromatography. Several peaks were obtained in each brucellin fractionation.The third criteria was immunization of 5 groups of guinea pigs .The fourth criteria was the using of serum of immunized animals in ELISA test against peaks resulted from fractionation . Peak 1 of Rev1 brucellins show the highest positive results and considered as the responsible part of immunization against brucellosis .