Effect Of Oocyte Harvesting Techniques on Recovery and on In Vitro Maturation in Iraqi Sheep

Abstract

Two different methods of recovery, aspiration and slicing were used for harvesting the oocytes of ovine ovaries. The aim was to determine an efficient method for oocyte harvesting and its effect on subsequent in vitro maturation. The average percentage of oocytes recovered by methods was significantly higher (p<0.05) by slicing (84.60± 1.01) than by the aspiration method (67.60 ± 1.37). However, the percentage of good quality mature oocytes was higher in the aspiration method (40.57 ± 0.90) as compared with slicing methods (37.40 ± 0.27). There was a significant difference (p<0.05) between the two methods.It was concluded from present work that the method of harvesting oocytes from ovaries in slaughter house effect on recovery and the maturation of the oocyte in sheep