Isolation of lactic acid bacteria (LAB) species as probiotic from intestinal contents of common carp Cyprinus carpio L.

Abstract

The presence of lactobacilli were investigated in the intestine of 50 fish specimens common carp cyprinus carpio weight rang between 400-1500g in weight from commercial farms in north of Baghdad through period of September to December 2010. All isolates were Gram positive ,catalase negative short rod usually non motile and non sporulating bacteria that produce lactic acid as a major or sole product of their fermentative metabolism.The obtained data showed that various species of lactic acid bacteria were found with high levels in total number about 104-107CFU/g of intestinal content, physiological and biochemical characteristics of 8 strains isolated from intestine J1, J3, J4, J6, J7, J8, J9, J10 that can be categorized in two metabolic group facultative and obligate heterofermentation ,also all isolates were resistante to all antibiotic sensitivity OA2A –p Disc except isolates J1, J3, J4, J8, J9 were sensitive to erythromycin in concentration 60mcg .The aim of this study was to characterize of lactic acid bacteria isolated from the gastrointestinal tract of common carp Cyprinus carpio to be used as probiotic.