A STUDY OF SOME HEMATOLOGICAL TRAITS DURING EXPERIMENTAL INFECTION WITH INFECTIOUS BURSAL DISEASE IN BROILER CHICKS

Abstract

The research was conducted to evaluate some hematological parameters during experimental infection with infectious bursal disease in broiler chickens . For this purpose 30 chicks were infected with the virus of the disease at 18 days old, whereas other 30 chicks were used as a control group . During the first and seventh day of the outbreak some hematological parameters were estimated which included packed cell volume , hemoglobin concentration and heterophil lymphocyte ratio as well as some biochemical tests such as serum protein , glucose, cholesterol , creatinine and uric acid , Aspartate Amino Transferase, Alanine Amino Transferase and Alkaline Phosphatase during the first and seventh day of infection with the virus. Results showed a significant reduction (P<0.05) of Packed Cell Volume (28.60%) and hemoglobin concentration (8.91gm100ml) in blood of infected birds and a significant increase (P<0.05) of HL (1.49) during the seventh day of infection . Infection caused a significant increase (P<0.05) of serum protein (10.14mg100ml) as well as a significant reduction (P<0.05) of serum glucose (168.60mg100ml) with a significant increase (P<0.05) of serum cholesterol (233.60mg100ml) during the seventh day of infection with the disease , whereas serum creatinine was not significantly differ between the infected and the control groups of chicks neither during the first nor the seventh day of infection , meanwhile , uric acid showed a high significant elevation (P<0.01) to become 10.80 mg100ml during the seventh day of infection . It was noticed that infection caused a significant elevation (P<0.05) of AST enzyme (23.0 IUL) during the first day of infection , however , ALT enzyme was not significantly differ between the infected and the control groups of chicks neither during the first nor the seventh day of infection , where as ALP enzyme was high significantly reduced (P<0.01) to become 60.80 and 63.40 IUL respectively during the first and seventh day of infection.