Assessment of the Immunogenic Potential of the Infectious Bursal Disease Vaccine Virus (G-61) in Broiler Chicks

Abstract

Using of numerous methods of vaccination of broiler chicks leading to variation in theaverage of immune response that elicited against Gumboro vaccine viruses. The study aimedto determine the effect of the immunogenicity of the live attenuated infectious bursal disease(IBD) vaccine virus (G-61 strain) in Broiler Chicks. A total of (160) chicks were divided intofour equal groups namely A, B, C and D (40 chicks per group). The chicks of groups(A, Band C) were vaccinated with live attenuated IBD(G-61) vaccine at (14) days old, via theaerosol, intranasal and drinking water routes respectively while chicks of group D were leftwithout vaccination as a control. An indirect enzyme linked immunosorbent assay (ELISA)was used to detect the antibodies titres against infectious bursal disease (IBD) vaccine virus insera of chicks at (28) days of age. At (29) day of age, chicks of all groups were challenged byusing of a virulent IBDV. At (40) day of age, the levels of antibodies against infectious bursaldisease virus have been measured by indirect ELISA. The results obtained showed that higherlevels of antibodies were noted when the vaccine administered via aerosol route as comparedto the intranasal and drinking water. Following challenge of vaccinated chicks, the protectionrates noted are correlated to the levels of antibodies elicited. It is concluded that the choice ofapproach to achieve higher and protective immune status against infectious bursal diseaseviruses in chicks is to apply IBDV (G-61) vaccine strain in broiler chicks via the aerosolroute.