Evaluation of the inhibitory effect of a number of medicinal plants fixed oils on a group of pathogenic bacteria invitro

Abstract

Evaluation of the inhibitory effect of a number of medicinal plants fixed oils on a group of pathogenic bacteria invitroZ. A. H. Al- EbadyColl. of Vet. Med./ Univ Al- QadisiyaAbstractThe present study was designed to evaluate the antibacterial effect of 4 of local medicinal plants oils :Eletteria cardaomum,Nigella sativa,Prunus dulcisvar and Brassica alba in addition to 5 Antibiotics: Ciprofloxacin ,Chloramphenicol, Aztereonam, Sulfamethaxazole /Trimethoprime and Erythromycine as a positive control against 7 gram positive and gram negative pathogenic bacteria:Micrococcus spp.,Staphylococcus cohnii cohnii ,Enterobacter cloacae,Escherichia coli, Proteus mirabilis,Pseudomonas aeroginosa and Klebsiella pneumonia by using of agar well diffusion method . The result showed that for the oil of Eletteria cardamomum the best antibacterial effect was produced against Proteus mirabilis followed by Klebsiella pneumonia then Enterobacter cloacae,Pseudomonas aeroginosa,Escherichia coli,Micrococcus spp. and Staphylococcus cohnii cohnii , and for the oil Nigella sativa the antibacterial effect was highest against Micrococcus spp., followed by Proteus mirabilis then Escherichia coli and Staphylococcus cohnii cohnii. Brassica alba oil express it is antibacterial effect only against Pseudomonas aeroginosa and there was no effect on any other bacteria while Brassica alba had no effect on any of the studied bacteria.The inhibition of the growth of Pseudomonas aeroginosa was stronger by Prunus dulcisvar oil than Eletteria cardamomum oil .For Escherichia coli,Staphylococcus cohnii cohnii and Micrococcus the wider zone of inhibition were obtained by Nigella sativa oil than Eletteria cardamomum oil, while for Proteus mirabilis the inhibition of the growth was higher for the oil of Eletteria cardamomum than Nigella sativa oil.The values were given as mean ±Standard deviation and P< 0.05 was considered statistically significant.The datad were analyzed by student's t-test using SPSS (version 10).