Effect of Plant extract of Nigilla Sativa on the effectiveness of the Parasite Entamoeba histolytica in Patients from Al-Hammar Marsh Iraq .Al-Nasiriyah.

Abstract

Stool samples from people which were suffering from acute diarrhea had been chosen At Al-Hammar Marsh and from reviewers of a clinic in the city of Al-Nasiriyah Chibaach during the month of June 2009 .Stool examination results showed that the proportion of total parasite infection was 49% Entamoeba histolytica Infections were higher in Males ,reaching 54,8 % compared with 54,2 % for females .To investigate the possibility of parasitic Amoeba growth Semi Solid Medium was very successful as a good media for that purpose .Using the oily extract of the black seed had an a good effect on amoebic Parasite killing for a short period in compare with Metronidazole drug ,especially with the dose of (0.04) ml ,results from using a mixture of a black seed oil and the enzyme of Cystein proteinase, Metronidazole and Tinodazole showed a great inhibition for the parasite enzymic activity to (34,48,50)ml, respectively. As a conclusion the results showed that black seed oil extract and these three drugs were very effective factors on the trophozoits phases of Entamoeba histolytica Parasite and the most effectiveness can be gaine from the black seed oil extract.