Study the effect of cadmium on some biochemical aspects of mature earthworms Aporrectodea caliginosa

Abstract

The research was conducted to study the effect of cadmium on some biochemical aspects of adult earth worms Aporrectodea caliginosa used for this perpuse five concentrations of cadmium which 1 , 3 , 5 , 7 , 9 ppm in addation to the control group . The worms were adminstrate with mentioned cadmium concentration for 14 days , then determined the leathal concentration and half leathal concentration . Also determined the biochemical effects by determine the proteins , transferases and alkaline phosphatase . Also determined the nature of protein bands by poly acrylamide gel electrophoresis . The results was :1. The leathal concentration was 9 ppm while the half leathal concentration was 7 ppm .2. Occur significant decrease in soluable protein concentration (p<0.05) in earth worm extraction compare with control group , at all study cadmium concentration 3 , 5 , 7 ppm , when used electrophoresis method for proteins show change in protein bands disappear some bands and increase density of some and decrease density of others.3. Occur significant increase (p<0.05) in Alanine Aminotransferase , Aspartate Aminotransferase and Alkaline phosphatase compare with control at all study cadmium concentrations .