The activity of different types of sera on the growth of trophozoite of Entamoeba histolytica in vitro

Abstract

The parasite Entamoeba histolytica has been isolated and cultivated in vitro via using Locke-egg medium (LEM) and Liver infusion agar medium (LIAM) . After that, the effect of some types of sera (sheep, bovine and human) on the growth and activity of the parasite in the two culture media was investigated. The reproduction rate has been significantly increased when the sera of sheep and bovine were supplemented to LEM medium to reach 105.5 and 142.6%, respectively. Human blood group (A) serum was also effective, but with a less degree, and such effect was a medium-dependent (LEM: 49.1%; LIAM: 26.9%).