تاثير استخدام مادتي الاكردين البرتقالي وصوديوم دودوسايل سلفيت في ازالة مقاومة جرثومة Staphylococcus aureus للمضادات الحيوية

Abstract

This study has involved the isolation of staphylococci bacteria (coagulase positive) from different clinical human sources of infections (blood, urine, pus, otitis, tonsils). These bacteria were diagnoized by using microscopic examination and the biochemical tests.Out of (119) isolates of staphylococci samples, (91) of them are related to S. aureus with percentage (76.5)% while the percentage (23.5)% represented the coagulase negative bacteria.These isolates of S. aureus was classified according to their resistance to the antibiotics (Ampicillin, Tetracycline, Chloramphenicol, Streptomycin, Gentamicin, Penicillin and Trimethoprium), as a result, there are five groups of isolates showing variation in their resistance to the antibiotic understudy.The two chemical materials are used to eliminate the antibiotics resistance of bacterial isolates understudy sodium dodecyl sulfate (SDS) appear to have high effectiveness in curing the plasmid DNA (nearly 100%) for most antibiotics then the dye acridine orange (A.O).The isolates (2) of bacteria from (obtitis abscess) show efficient respond for elimination their antibiotic resistance by the two chemical materials as it is compared with other isolates.-lactam plasmid of the isolates bacteria appear to be more affected resistance in curing experiments.