Spectral Characteristics of Cholesterol in Blood by Using Simple Semiconductor Laser


In the present work, the possibility of using laser to analyze and find the concentration of total cholesterol (TC) in serum. Semiconductor laser diode of 5mW maximum power, 532nm wavelength and 4nm bandwidth is used to accomplish assay measurements. The spectral plot showed that the maximum absorbance of cholesterol is at the range (470-540nm) and the peak of spectral absorbance centers at 500nm.The laser system measurements include the study of absorbance as a function of both laser power and sample thickness, as well as the transmittance as a function of Laser power. To ascertain our calculations, the results have been compared with the results of spectrophotometer. The RSD values between them are about (1.7-9.8).