Determination of Inorganic Phosphorus in Human Blood Serum by use Square Wave Voltammetry (SWV)


AbstractA square wave voltammetric method is described for the determination of Inorganic Phosphorus in human blood serum. This method is based on the decrease on the reduction peak of ferrous ammonium sulphate Fe(NH4)2(SO4)2.6H2O appeared at (-0.2 V) vs. (Ag/AgCl,3M KCl) as a reference electrode using (Sodium acetate, acetic acid) buffer adjusted by (1.0 N) NaOH to (pH 7.0) as supporting electrolyte due to its consumption via the enzymatic reaction of Inorganic Phosphorus. The method was successfully applied to measure the Inorganic Phosphorus in various blood samples represent different cases such as: hyperparathyroidism, rickets (in children), osteomalacia (in adults), chronic renal failure, Addison’s disease and diabetic ketoacidosis.The results have been compared with those obtained from the spectrophotometric method and show a good agreement between the two methods with correlation coefficient (r = 0.9996).