Regeneration of date palm (Phoenix dactylifera L.) CV. Sherafy from different apical explants In vitro

Abstract

This study has been performed to determine the response of apicalsources [shoot tip ; Auxillary buds ; sub apical tissues ; leaf primordial andflower buds] when cultivated on MS medium supplemented with 30 mg/L ofNAA ; 3 mg/L of 2ip and 3 g/L activated charcoal in inducing ; formation ofcallus and production of somatic embryos on the same medium enriched with10gm/L of NAA ; 2 mg/L of 2ip and 2 g/L activated charcoal and formationorganogenesis from callus on two media which were supplemented with 3 mg/L2ip ; 1 mg/L 2ip and 1 mg/L NAA ; 250 mg/L charcoal for each media.Results revealed that the flower buds surpassed in the time requested forcallus estabelish ment and the plant sources ratio which produced the callus, withsignificant difference than other explant, the sub apical tissue failed to producethe callus also, the shoot tip treatment had the highest average of produced callusthan other explant. Statistical analysis proved that the difference between flowerbuds and shoot tip was not significant in the parameter of the dry weight ofcallus. Which cultivated in ms medium supplemented with 10 gm/L of NAAafter two months from culturing, while the dry weight decreased significantly inleaf primordial than other explant.Flower buds treatment had the optimam periode to estabelish thecylinderial embryos and number in comparision with other explant while thelongest periode recorded in leaf primordial treatment.Resulted showed that the callus cultured on the medium supplementedwith 3 mg/L of 2ip decreased significantly in the requested periode fororganogenesis growth which were (186 and 213.25) days respectively.The interaction between (shoot tip and 3 mg/L of 2-ip) was surpassed theexplant of shoot tip and 1 mg/L of 2ip in the requested time for appearance oforganogenesis, also some individual cylindrical somative embryos developedfrom the callus cultured on the above media with some secondary embryos whencultured on the same medium produced muttistem plantlets .