ISOLATION AND IDENTIFICATION OF SOME LOCAL DATE USING GEL FILTRATION CHROMATOGRAPHY & ELECTROPHORESIS

Abstract

The present study is concerned with studying physical & chemical examinations fordates & seeds of some local varieties such as: Hallawi, Sayer & Barhee. The resultsof the statistical analysis about the physical properties showed that there were highsignificant differences among varieties in weight, diameter, size, flesh weight & seedweight characters, but the date length did not appear any significant differencesamong the varieties.T he results of the chemical composition of destoned dates showed that Barheevariety was highest in content of moisture & fat whom reached 31.43 & 0.6%respectively & with high significant differences compare with varieties of Hallawi &Sayer, while Sayer variety showed higher content of ash which reached 2.3%, whileprotein content did not show any significant differences among the varieties.Moreover, the results of the protein extraction by different solvents for dateshowed that the water soluble proteins (albumins) was highest which was about 46.28- 65.07%, while was lower to salt soluble proteins (globulins) which was about 4.04 -6.03% from the total amount of proteins.G el filtration for water soluble proteins which extracted from date showed thatexistence three major peaks, while salt soluble proteins showed existence two majorpeak with one minority peak between them, for all varieties. In case of alcoholsoluble proteins (prolamins) demonstrate existence small peak followed by onemajority big peak, while alkali soluble proteins (glutenins) showed one majority bigpeak between two small peaks.Electrophoresis studies showed that the salt soluble proteins extracted from date:Hallawi, Sayer & Barhee, appearance three major bands whom were molecularweights about 61000-62400 Dalton for first band, 27600-37500 Dalton for secondband & 18200-23500 Dalton for third band.