Isolation and diagnose of fungi associated with the cultivation of tissues of five cultivars of date palm (Phoenix dactylifera L.) and the effect of fungicide Benlate in control .

Abstract

this study was Carried during the growing season 2009 in the laboratories of the Date Palm Research Center at the University of Basra, the aim of this study to isolate and diagnose fungi associated with the cultivation of date palm tissue and the effect of fungicide Benlate in control . Where isolated and diagnosis of several species fungal of five different cultivars of date palm (Quantar , Auwaidy , Khadrawi , Hillawi and Brahi). Where record two types of fungus Chaetomium on explants of the Auwaidy cultivar (c. atrobrunnenm and C. globosum) and record type (C. Strumarium) on Khadrawi cultivar . Recorded presence of two types of the fungus Aspergillus sp. On Quantar cultivar and another type of fungi on Auwaidy cultivar . Fungi Aspergillus niger on Khadrawi cultivar . Also recorded three types of the fungus Fusarium sp. on cultivars (Quantar, Auwaidy and Khadrawi) separately.Wheras the flower buds were recorded fungi (Aspergillus sp. .F.solani and Gilmaniella humicola) on the Halawi cultivar. And recorded two types of the fungus Alternaria sp. On the Barhi cultivar . The study also demonstrated that dipping explants (Apical buds, axillary buds, leafs primordial and flower buds) in Benlate with concentrations (75 and 150 mg / L) for three minutes contributed to the reduction of pollution fungi compared with non-immersion (treatment control). The percentage of contamination decreased to (4.16% , 4.16% , 0.0% ,0.0% and 4.16%) at Benlate 150 mg / L, for cultivars Quantar , Auwaidy, Khadrawi , Hillawi and Brahi respectively compared to the treatment of control (non-immersion) that the percentage of pollution fungi which (29.16% and 37.5% and 20.83% and 33.33% and 41.66% for five cultivars under study respectively.