The source of Bacterial Contamination in Date Palm (Phoenix dactylifera L.) Grown Invitro

Abstract

This study was conducted to determine types of bacteria contaminating three types of callus of date palm cultivars (Al-sayer,Al-kentar, Albream ) and comparated with types of bacteria that isolated from shoot tips of healthy offsets of date palm grown in tissue culture media .Four genera of bacteria were identified from contamination detected (24-48h )after grown culture (External contamination )these are ;(Bacillus 39% ,Staphylococcus 31% ,Escheritia 21% , Serratia 8%) while Bacillus was the only genus isolated from contamination detected (4-8 weeks)after growing cultures``(internal contamination ) . identification of genus Bacillus showed the prevalence of B subtillus from External contamination and the species B circulans from internal contamination and shoot tips of healthy offsets of date palm . B circulans that isolated from internal contamination and shoot tips of healthy offsets of date palm showed sensitivity to antibiotic Setreptomycin at the concentration 0.75 while the antibiotic Gentamycin showed inhibiting activity at the concentration 0.50 agianest the species B circulans .