Effect of Laser Radiation on Propagation of Date Palm Phoenix dactylifera L. Cv. Barhi In vitro

Abstract

This experiment was conducted to find the effect of exposure period of Laser radiation (10,20,30) second and (0)as a control treatment, distance (1,2)cm. at wave length(630-660)nm and power 2mw on embryogenic callus and somatic embryos of date palm Cv. Berhi.Results showed that the Laser treatments had a significant effect on callus fresh and dry weight ,somatic embryos number., embryos lengths ,germination percentage. leave length, root lengths ,fresh and dry weight of plantlets, recorded 1.324g by 20 second.,0.086g,by 30 second , 13.830embryos by 10 second , 5.500mlm by 10 second , 45.270% by 10 second , 1.324 by 10 second , 6.760cm by 20 second , 1.491gm by 30 second , 0.131g by 30 second , respectively.Also the treatment by 2 cm. was the best effect on callus fresh weight , somatic embryos no. leave lengths ,and plantlets dry weight recorded 1.150gm, 12.080 embryos, 1.200cm, 0.110gm respectively