Effect of Viola odorata extract on Pseudomonas aeruginosa produce β-lactamase enzyme


Background: Pseudomonas aeruginosa is a classic opportunist pathogen with innate antibiotics and disinfectant. It is physiologically versatile and flourishes as a saprophyte in warm moist situation within human environment, including sinks, drains, respirators, humidifiers and disinfectant solutions.Aims: investigate the antibacterial activity of Viola odorata extract in vitro.Patients and methods: alcohol solvent was mainly utilized for such extraction procedure with subsequent application of the extract against resistance Pseudomonas aeruginosa isolating from different body sites. Twenty two isolates, out of 222 samples, produced Beta lactamase (β-lactamase) and such isolated bacteria was examined for antibiotic sensitivity test toward ten antibiotics namely ampicillin, Augmentin, cefotaxime, amikacin, gentamicin, ciprofloxacin, tetracycline, trimethoprim, tobramycin, and imipenem. Results: it was revealed that the alcohol extracts of Viola Odorata; range with half concentration (500, 250, 125, 62.5, 31.25, 15.7 mg/ml); exhibited broader spectrum as well as greater activity against resistant Pseudomonas aeruginosa among those with/without tested extracts with inhibition zone vary between (10-30 mm). Conclusions: These data suggest that Viola odorata extract could inhibit the growth of Pseudomonas aeruginosa strain in vitro and this activity may contribute to its chemopreventive effect.