THE ROLE OF REACTIVE OXYGEN SPECIES IN THE CHEMILUMINESCENCE REACTION OF

Abstract

The oxidation of luminol by hypochlorite to generate chemiluminescence (CL) has beenstudied by using a multipurpose photon counting system. The processes of light production ,which invovle the reactive oxygen species (ROS), are discussed in terms of the NaOCl-Luminolreaction .The addition of radical scavengers such as ascorbic acid and dimethylsulfoxide (DMSO) leadto decreases in the peak of CL reaction of both system NaOCl-Luminol and FeSO4 – Luminolrespectively, while the addition of persulfate lead to increase in the peak of CL. The resultssuggest that ROS radicals might be constant factors in CL reaction of NaOCl-Luminol system