INDUCING RESISTANCE AGAINST RHIZOCTONIA SOLAN I USING AZOTOBACTER CHROOCOCUM AND TRICHODERMA HARZIANUM IN POTATO PLANTS

Abstract

In an experiment conducted at soil science and water resource department/College of Agriculture to investigate the induce resistance in potato plants against Rhizoctonia solani. Azotobacter chroococum and Trichoderma harizanum were isolated and identified from farm soil located at Baghdad and Anbar province to be used individually or together to assess their effect on Rhizoctonia solani the causal agent of black scurf disease of potato in a greenhouse experiment according to CRD design. Analysis of plant leaves protein showed two bands with molecular weight of 32.00 and 29.00 KD in plants treated with R. solani and disease severity 3.54 for the stem, 3.53 for tubers. One protein band with 38.00 KD mwt was observed in T. harizanum and R. solani with disease severity 1.00 for stem, 0.71 for tubers. Two protein bands with 29.00, 32.00 KD mwt were appeared in A. chroococum and R. solani treatment with disease severity 0.71 for stems and tubers. Three protein bands 38.00, 40.00, 42.00 KD mwt were appeared in T. harizanum and A. chroococum and R. solani with disease severity 1.6 for stems and 0.71 for tubers.