EVALUATE EFFECTIVETY OF DIFFERENT ISOLATE OF PSEUDOMONAS FLUORESCENSE AGAINST FUSARIUM OXYSPORUM AND RHIZOCTONIA SOLANI

Abstract

This study was conducted in plant protection department Agriculture College and Marin Center University of Basrah 25 isolates of Psedomonas fluorescens were obtained from different geographical area. The isolate were identified according to their biochemical profile and differences between five isolate were done by RAPD-PCR .Results of antagonism test revealed that pfm3 and pfm5 were more effective against Fusarium oxysporum and Rhizoctonia solani as radial growth reach 2.7And 2.9 cm respectively compared to 9 cm in control treatment. Additional experiment showed that bacterial isolate reduced the negative effect of the pathogenic fungi as seed germination of Okra increased from 20 and 23.3% in F.oxysporum and R.solani to 86.6 and 83.3 respectively. Result also showed that bacterial isolate were effective in reducing okra seedling damping-of which reduced from 83.3 and 86.6 in F.oxysporum and R.solani(control treatment) to 13.7 and 10.3 in soil treated with pfm3 isolate.