Detection of Human Cytomegalovirus (HCMV) among Infertile Male in AL-Najaf governorate

Abstract

The current study aimed to detect human cytomegalovirus among infertile individual. Bloodand seminal fluid collected from 300 infertile male randomly , ELISA technique was used to know theactivity of humoral immunity among them through measuring anti-HCMV IgM and IgG antibodies ,the result revealed that 31 (11%) were positive for IgM result and 234 (78%) were positive for IgG.The PCR technique showed that out of 300 samples only 22 (7.4%) gave positive results,In case of control group, results were negative for anti- HCMV IgM antibodies in ELISA testand PCR technique. While IgG gave a weak positive results.Aim of the study:The present study was to study the relationship between the virus and infertility throughdetection of human cytomegalovirus (HCMV) infection in infertile male in AL-Najaf governorate byscreening of anti-human cytomegalovirus IgM and IgG in the serum and detection of humancytomegalovirus DNA in seminal fluid, using PCR technique.