Effects of Gold and Silver Colloidal on Gama Glutamate Transferase Enzyme Activity in Blood Serum

Abstract

Laser ablation of metal plate in water was performed in order to obtain gold and silvernanoparticles. Transmittance electron microscopy TEM and uv-vis spectrophotometerwere conducted in order to determine the size and optical properties of the nanoparticles,respectively. The nanoparticles concentrations were also characterized by atomicabsorption spectroscopy AAS measurement. The absorbance spectra show a sharp andpeaks around 400 or 525 nm, indicating the production of silver and gold nanoparticleswith average size of 25 nm, have been confirmed by TEM. Both Gold and silvernanoparticles show inhibition on the gama glutamate transferase (γ-GT) activity. Theinhibition effect increase as a function of nanoparticles concentrations. Kinetic propertiesof (γ-GT) activity revealed (by nanoparticles) mixed type of inhibition.