Comparative Study of Cellular Immune Response in Cutaneous Blastomycosis

Abstract

Background: The immune response against Blastomyces dermatiditis is lessclear than that of Histoplasma spp. or Coccidioides spp. This study was done toevaluate the cellular immune host response in patients with ulcerative andverrucous skin lesions.Methods: This study was conducted on 50 patient attended Al-KadhymiahTeaching Hospital/Dermatology Dep. in Baghdad (2009-2010). After processing ofskin samples, all cases were studied for various lymphocyte subtypes by usingdirect dual immunofluorescence staining method. Independent sample t-test wasused to make a comparison between groups and p-value <0.05 was consideredsignificant.Results: Histopathological examination showed 4 biopsies of skin lesions wereBlastomyces dermatitidis positive and 46 biopsies were negative, two cases wereulcerative and two were verrocous . According to this classification, the total cellcounts, CD3+ cells was found statistically different (p=0.034). Plasma cell (CD19),T-helper (CD4) and T-cytotoxic (CD8) also were significant when p values were(p=0.046), (p=0.020) and (p=0.018) respectively. According to the type of lesionwhether it is ulcerative or verrocous, CD3+, CD4+, CD8+ and CD19+ counts foreach type were significant when, P value were (p≤0.001, p=0.003, p=0.026 and0.050) respectively. However, there were no significant difference was seen forthese cells (p>0.05) among negative lesions.