Detection of Brucella in infected bull testis by using PCR technique

Abstract

Brucella infect male animals and cause orchitis and infertility, present study aimed to isolate brucella from testes of bull, 50 testes samples were collected then withdraw sample from epidedimys after that stained with modifiedziehl neelsen, samples were positive for stain were cultured on brucella selective media and tested with PCR. Results showed 6 samples were positive for modified ziehl neelsen , 4 samples were positive for culture and 6 samples were positive for PCR,from our research showed that PCR is more effective in diagnose Brucella infection.