تعيين الظروف المثلى لانتاج انزيم alginase من بكتريا Bacillus sp. المعزولة من التربة

Abstract

Twenty fife isolates belonged to Bacillus sp. were obtained from fifty samples from soil collected from different areas in Baekoba/ Diala governorate. Ability of alginase production by these isolates was screened. Bacillus sp. S7 isolate was the highest alginase producer. Alginase production conditions by Bacillus sp. S7 were studied, the highest production of alginase was observed where sodium alginate broth containing 1% nutrient broth in pH 8.5 inoculated with 0.2% of bacterial cells and incubated at 35oC in shaking incubator 120 rpm for 18 hr. The effect of some amino acids and salts on alginase production were studied and found that 0.1% of asparagine and 0.4% of NaCl increased the enzyme productivity, while KCl has no effect on its productivity.