A Multiplex PCR for Detection of hlyA, papC, and traT genes in multidrug resistance Escherichia coli isolated from pregnant women

Abstract

Abstract:Urinary tract infection (UTI) in pregnancy is a common clinical problem which causesmorbidity and, in a small minority of cases, renal damage and chronic renal failure. Urinesamples from three hundred pregnant women were collected from Baghdad TeachingHospital, were examined between January to June 2012 for the presence of bacteria. Theirages ranging from 15 to 50 years. Up to 32.6% (98) of women have a urinary tract infection(UTI). Escherichia coli was detected in 27.5% (27) of all the urine samples.All isolates were screened for some virulence factors: α-haemolysin, adhesion factortype P fimbriae and serum resistance. Multiplex PCR systems were used to detect genesencoding α-hemolysin (hlyA), adhesion P(papC), and traT associated with serum resistance.The results indicated that the occurrence of papC was detected in (18.5%), while traT wasdetected in (96.2%) of isolates on the other hand hlyA can't detected in any E. coli isolates. Allthe isolates were also studied for antibiotic susceptibility pattern using a disc diffusionmethod.The rate of resistance of E. coli Amoxicillin (92.5%), Amoxicillin/Clavulanic acid(89%), Cefotazidime, Cefotaxime and Cefepime (78%), kanamycin (59%), levofloxacin(55.5%), Ciprofloxacin (52%) and showed high degree of sensitivity toward Cefoxitin andImipenem (7% and 0%) respectively. Additionally,17 (62.9%) isolates were multidrugresistant.The finding of this study indicated that the traT widespread among pathogenic E. coliin Iraq. Furthermore it concludes that E. coli is one of the important multidrug resistancecausative agents of urinary tract infection in young women especially during the state ofpregnancy.