Usage of local propolis formulae for treatment of mastitis in ewes

Abstract

The aim of this research was to use of ethanol extract Local propolis (EEP) in treatment of mastitis in ewes Locally as Intra-mammary Infusion To carry out this aim , Survey was made for mastitis in the animal fields of veterinary and Agriculture college of Baghdad University ,Where 88 ewes were examined and 168 milk samples were collected , and the results show that : The percentage of clinical mastitis was 5.11% while the percentage of subclinical mastitis was 14.20% from the total udder halves that examined , Thirty five bacterial strains were isolated from clinical and subclinical mastitis in ewes , After confirmed each strain by morphological and biochemical Characteristics , Staphylococcus aureus was the most prevalent pathogens in the clinical mastitis (44.44%) while Staphylococcus epidermidis was the most prevalent pathogens in the subclinical mastitis (53.84%), Staphylococcus aureus was the most pathogens causing elevating of Somatic Cell Count in ewe’s milk , also the result showed that Direct Microscopic Somatic Cell Count was more confident than California Mastitis Test in detection of subclinical mastitis in ewes , In addition to isolate Pseudomonas aeruginosa for the first time in Iraq compared with previous studies. This Propolis Ethanolic Extract show antibacterial activity against Staphylococcus aureus in Vitro by Agar Diffusion Method. The antibacterial activity of propolis preparation 1% were proved clinically with bacterial Cure in 3 cases of ewes naturally infected with subclinical mastitis caused by Staphylococcus aureus and mixed infection between Streptococcus uberis and Staphylococcus epidermidis .